| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 345 | WILLIAMS, Mitheron | JR | Boca Ciega | 52.56 | St. Petersburg HS Championship |
| 2 | 133 | MOSOKE, Brainharris | SR | Clearwater | 52.18 | 57th Annual Ed Wells Jr.-Kiwanis Invitational |
| 3 | 1088 | BJELLAND, Jackson | SR | Palm Harbor University | 50.92 | 57th Annual Ed Wells Jr.-Kiwanis Invitational |
| 4 | 284 | SPANN JR., Gregory | JR | Lakewood | 49.63 | Kowboy Invitational 2019 |
| 5 | 877 | FORDHAM, Jakir | SR | Countryside | 50.87 | 57th Annual Ed Wells Jr.-Kiwanis Invitational |
| 6 | 406 | WARE, Donquan | JR | Boca Ciega | 51.59 | Mustang Invitational |
| 7 | 959 | WALSH, Jake | SR | East Lake | 52.37 | East Lake Early Bird Invitational |
| 8 | 1190 | HINTON, Jervon | FR | St. Petersburg | 52.58 | West Coast Invite |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 91 | NEWSOME, Malik | JR | Seminole | 53.58 | Indian Rocks Cavonis Invitational |
| 2 | 863 | LOPEZ, Leroy | SO | Countryside | 53.27 | George M. Steinbrenner Track and Field Invitational 2019 |
| 3 | 375 | LAWES, Richardo | SO | Boca Ciega | 52.99 | Richard Allen Invitational |
| 4 | 1119 | WALSER, Connor | SO | Palm Harbor University | 52.80 | George M. Steinbrenner Track and Field Invitational 2019 |
| 5 | 73 | HULSEBOSCH, Sheldon | SR | Seminole | 52.82 | Calvary Christian Warrior Invite 2019 |
| 6 | 1192 | WILLIAMS, Malik | SO | St. Petersburg | 53.11 | 57th Annual Ed Wells Jr.-Kiwanis Invitational |
| 7 | 854 | GRIFFIN, Jaden | FR | Countryside | 53.40 | George M. Steinbrenner Track and Field Invitational 2019 |
| 8 | 312 | MAYBELL, Avion | JR | Lakewood | 53.59 | Kowboy Invitational 2019 |