| Athlete | Yr. | Team | Seed | Note | |
|---|---|---|---|---|---|
| 1 | HARRIS, Adrian | Lightning Bolt Track Club | 48.77 | ||
| 2 | JACKSON, Keandre | Polk County Elite | 49.00 | ||
| 3 | WILLIAMS, Mytherion | Lightning Bolt Track Club | 51.06 | ||
| 4 | FLOOD, Weldon | Monstarz | 53.00 | ||
| 5 | DURASTANTI, Zackary | Hardee Sole Crushers | 55.00 | ||
| 6 | BERNHEIM, Michael | Unattached | 55.60 | ||
| 7 | REEVES, Colby | Pure Athletics Ntc | NT | ||
| 8 | LOVEJOY, Jamarie | Pure Athletics Ntc | NT | ||
| 9 | VESTA, Brian | Olympia Track Club | NT | ||
| 10 | SMITH, Cameron | Victory Fitness | NT |