| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 582 | LOVEJOY, Jamarie | Pure Athletics Ntc | NT | ||
| 2 | 499 | RASHEED, Elijah | Polk County Elite | 24.60 | ||
| 3 | 418 | FLOOD, Weldon | Monstarz | 24.00 | ||
| 4 | 377 | HARRIS, Adrian | Lightning Bolt Track Club | 21.56 | ||
| 5 | 387 | WILLIAMS, Mytherion | Lightning Bolt Track Club | 22.12 | ||
| 6 | 586 | ROSSER, Luke | Pure Athletics Ntc | 24.50 | ||
| 7 | 261 | RUFFIN, Marcus | Florida City Track Club | NT |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 3 | 843 | SMITH, Cameron | Victory Fitness | NT | ||
| 4 | 41 | HANNON, Christian | AMP Athletics Track Club | NT | ||
| 5 | 584 | REEVES, Colby | Pure Athletics Ntc | NT |