| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 564 | DICKO, Tyler | Prodigy Athletics Track Club | NT | ||
| 2 | 444 | LITTLE, Joshua | Olympia Track Club | 2:50.00 | ||
| 3 | 295 | FRANCOIS, Cameron | Hardee Sole Crushers | 3:15.00 | ||
| 4 | 319 | TRAVIS JR., Jason | HORNET ELITE | 2:54.00 | ||
| 5 | 801 | CHARLTON, Zyen | Unattached | 2:45.00 | ||
| 6 | 257 | HALL, Marquez | Florida City Track Club | NT | ||
| 7 | 198 | GEORGIEV, Daniel | Central Florida Gliders | NT | ||
| 8 | 181 | PABON, Nessiel | Blazing Speed Track Club | 2:51.00 | ||
| 9 | 58 | THOMAS, Messiah | AMP Athletics Track Club | NT |