| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 22 | HOLLAND, Tania | 352 Track Club | 26.60 | ||
| 2 | 250 | MILLEDGE, Alani | Fast Lane TC | 26.00 | ||
| 3 | 423 | CHRISTOPHER, Brianna | Monstarz | 25.00 | ||
| 4 | 515 | HART, Paris | Polk county express | 23.00 | ||
| 5 | 837 | JOHNSON, Twaneise | V12 Athletics | 24.60 | ||
| 6 | 251 | STCYR, Destiny | Fast Lane TC | 25.70 | ||
| 7 | 704 | SMALL, Alexis | Speed Academy | 26.03 | ||
| 8 | 757 | KING, Nischa | Take Flight Athletics | 26.70 |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 70 | JOHNSON, Faith | AMP Athletics Track Club | NT | ||
| 2 | 760 | WATSON-BELL, Allyson | Take Flight Athletics | 28.40 | ||
| 3 | 659 | RANDELL, Nia | Running Tigers Track Club | 27.50 | ||
| 4 | 702 | HEMMINGS, Dinae | Speed Academy | 26.80 | ||
| 5 | 24 | OWENS, Jasheena | 352 Track Club | 26.90 | ||
| 6 | 827 | LABORD, Morgan | Unattached | 28.00 | ||
| 7 | 130 | FREDERICK, Jermarrha | Ballistic Track And Field | NT | ||
| 8 | 652 | JEFFERY, Jayla | Running Tigers Track Club | NT |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 654 | LEWIS, Trinitee | Running Tigers Track Club | NT | ||
| 2 | 73 | NEBEL, Isabella | AMP Athletics Track Club | NT | ||
| 3 | 23 | LANCE, Jada | 352 Track Club | NT | ||
| 4 | 67 | HILL, Kamorri | AMP Athletics Track Club | NT | ||
| 5 | 63 | DAVIS, Leeiah | AMP Athletics Track Club | NT | ||
| 6 | 721 | TALBERT, Cecilia | Speed Starz Track/Running Club | NT | ||
| 7 | 136 | HENDERSON, Erica | Ballistic Track And Field | NT |