| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | GONZALEZ, Ashley | George Jenkins | 52.17 |
| 2 | WALKER, Dezha | Osceola (Kissimmee) | 51.67 |
| 3 | MASON, Caroline | George Jenkins | 49.10 |
| 4 | PHILLIPS, Amaya | Osceola (Kissimmee) | 45.58 |
| 5 | HUYKE, Helena | Celebration | 46.54 |
| 6 | GIGGETTS, Simone | St. Cloud | 50.19 |
| 7 | WOOD, Abigail | George Jenkins | 52.08 |
| 8 | SHIRLEY, Naomi | Osceola (Kissimmee) | 53.59 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | LINK, Tre'Keirriea | Kathleen | 55.48 | 55.48 Polk County Championship 2019 (3/16/2019) |
| 2 | HOGG, Jaylyn | Kathleen | 53.68 | 53.68 George Jenkins Invitational (2/16/2019) |
| 3 | BARR, Heaven | George Jenkins | 51.41 | 51.41 George Jenkins Senior Night (4/03/2019) |
| 4 | PHILLIPS, Amaya | Osceola (Kissimmee) | 45.61 | 45.61 Spruce Creek George Janan Invitational (3/02/2019) |
| 5 | MASON, Caroline | George Jenkins | 49.49 | 49.49 George Jenkins Senior Night (4/03/2019) |
| 6 | WOOD, Abigail | George Jenkins | 52.68 | 52.68 George Jenkins Senior Night (4/03/2019) |
| 7 | ANDINO, Haley | Tohopekaliga (Kissimmeee) | 54.63 | 54.63 Orange Belt Conference Championships (4/04/2019) |
| 8 | CLARKE, Umayah | Kathleen | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | TUFINO, Keisey | Ridge Community | 56.70 | 56.70 Polk County Championship 2019 (3/16/2019) |
| 2 | HOGG, Ashlyn | Kathleen | 53.79 | 53.79 George M. Steinbrenner Track and Field Invitational 2019 (3/30/2019) |
| 3 | WALKER, Dezha | Osceola (Kissimmee) | 51.42 | 51.42 Orange Belt Conference Championships (4/04/2019) |
| 4 | HUYKE, Helena | Celebration | 45.70 | 45.70 Miami Hurricane Invitational (3/15/2019) |
| 5 | GIGGETTS, Simone | St. Cloud | 49.45 | 49.45 Orange Belt Conference Championships (4/04/2019) |
| 6 | GONZALEZ, Ashley | George Jenkins | 52.56 | 52.56 Lakeland City Meet 2019 (3/07/2019) |
| 7 | SHIRLEY, Naomi | Osceola (Kissimmee) | 54.26 | 54.26 Storm Preview (2/14/2019) |
| 8 | PECK, Madeline | Celebration | 56.73 | 56.73 Orange Belt Conference Championships (4/04/2019) |