| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Daytona Express Inc | A | Oxendine, Jameel | Jones, Jeremy | Porter, Jordan | Rickerson, Kenton | DNF | |
| 4 | Space Coast Striders | A | Brown, Jaylen | Williams, Jesiah | Parker, Walter | Holmes, Xavier | NT | |
| 5 | Power House Athletics Track & | A | Gibson, Brandon | DNS | ||||
| 6 | Speed Academy | A | NT |