| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 892 | LE, Kevin | Wind Speed Athletics | 56.21 | ||
| 3 | 226 | MCGRIFF, Jacobe | Empire Athletics | 52.33 | ||
| 4 | 894 | ONUGHA, Godfrey Ike | Wind Speed Athletics | 51.92 | ||
| 5 | 430 | JOYNER, Jermaine | Peak Performance Track Club (P | 52.00 | ||
| 6 | 893 | LYFORD, Jonah | Wind Speed Athletics | 52.55 | ||
| 7 | 865 | LLERAS, Oscar | VAST Track Club | NT |