| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 421 | WOODS, Iyanna | Palatka Gliders | NT | ||
| 2 | 490 | LUKE, Shanya | Phase 1 Track Club, Inc. | 1:02.00 | ||
| 3 | 553 | CROKER, Zy'Kerriah | Power House Athletics Track & | 1:00.00 | ||
| 4 | 318 | GAMBLE, Haylee | FLORIDA EAST COAST SUPERSONICS | 57.00 | ||
| 5 | 885 | BLAKE, Brazyl | Victory Fitness | 1:00.00 | ||
| 6 | 845 | HUFFMAN, Amber | Unattached | 1:01.25 | ||
| 7 | 898 | SPELLS, Jahliyah | Wind Speed Athletics | 1:04.31 |