| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 16 | 440 | KING, Maniah | Peak Performance Track Club (P | NT | ||
| 17 | 286 | ROBINSON, Kariah | Epitome | 3:30.65 | ||
| 18 | 633 | HENRIQUES, Suhmer | RealDealRunners407 | 2:44.54 | ||
| 19 | 504 | WINGFIELD, Jaliah | Phase 1 Track Club, Inc. | NT | ||
| 20 | 699 | OLSEN, Madelynn | St. Augustine Speed TC | 3:15.00 | ||
| 21 | 69 | EIDE, Madeline | Central Florida Gliders | NT | ||
| 22 | 165 | WATSON, Nevaeh | Drapetomaniacs | 3:05.00 | ||
| 23 | 288 | SMOTHERS, Maleah | Epitome | 3:04.05 | ||
| 24 | 607 | PRESSLEY, Tianna | QUANTUM TRACK CLUB | 3:02.98 | ||
| 25 | 715 | WILLIAMS, Jakhia | Supreme Athletics Track Club | 3:06.00 | ||
| 26 | 65 | CLAUDIO, Makayla | Central Florida Gliders | 2:45.00 |