| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 10 | 409 | BLACKBURN, Jaylen | Ocala Cougars | 3:05.00 | ||
| 11 | 423 | BOYD, Kevin | Peak Performance Track Club (P | 3:00.00 | ||
| 12 | 433 | PERRY, Junior | Peak Performance Track Club (P | 3:00.00 | ||
| 13 | 51 | GEORGIEV, Daniel | Central Florida Gliders | 3:01.00 | ||
| 14 | 11 | PABON, Nessiel | Blazing Speed Track Club | 2:47.00 | ||
| 15 | 313 | WOOD, Dash | FLORIDA EAST COAST SUPERSONICS | 3:10.68 | ||
| 16 | 145 | WILLIAMS, Tyree | Daytona Express Inc | NT | ||
| 17 | 307 | NEESMITH, King | FLORIDA EAST COAST SUPERSONICS | NT |