| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Somerset Canyons | A | Noel, Jayden | Lentz, Sarah | Larose, Staicha | Ortega, Sarah | 4:32.52 | FHSAA 2A District 14 (4/08/2019) |
| 2 | Gulliver Prep | A | Brown, Nalani | Hall, Joy | Starvaggi, Nina | Varela, Natalie | DNF | FHSAA 2A District 16 (4/11/2019) |
| 3 | Lincoln Park | A | Senior, Nya | Clark, Amber | McCann, Christen | Wright, Chloe | 4:16.07 | FHSAA 2A District 13 (4/12/2019) |
| 4 | Glades Central | A | Shannon, Shaniah | Vickers, Patrianna | Louissaint, Ti'Uana | Mussenden, Rinaja | 4:11.36 | FHSAA 2A District 14 (4/08/2019) |
| 5 | Miami Edison | A | Joseph, Makyra | Massena, Marie | Cooper, Charkeria | Pierre, Choomay | 4:15.36 | FHSAA 2A District 16 (4/11/2019) |
| 6 | Astronaut | A | Jackson, Tigan | Ostopovich, Mia | Stouch, Alexandria | Joiner, Akiyonna | DNS | FHSAA 2A District 13 (4/12/2019) |
| 7 | Key West | A | Barter, Megan | Llama, Talissa | Matysik, Nicole | Niles, Julia | 4:23.21 | FHSAA 2A District 16 (4/11/2019) |
| 8 | Dreyfoos | A | Artero, Emma | Hansen, Amber | Vargas, Sasha | Haim, Vivien | 4:37.56 | FHSAA 2A District 14 (4/08/2019) |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ransom Everglades | A | Claybrook, Vashti | Scott, Brooke | Atabaki, Natalia | Tacher, Nicole | DNS | FHSAA 2A District 16 (4/11/2019) |
| 2 | Pine Crest | A | Torregrosa, Libby | Brown, Elizabeth | Schwartz, Amanda | Clark, Ashleigh | 4:00.42 | FHSAA 2A District 15 (4/13/2019) |
| 3 | Hallandale | A | Williams, Kiah | Castillo, Destiny | Walden, Janiya | Harvey, Ashantae | 3:54.57 | FHSAA 2A District 15 (4/13/2019) |
| 4 | Cocoa | A | Knowles, Domanique | Spann, Daishon | Thomas, Shaniya | Folston, Jatana | 3:51.26 | FHSAA 2A District 13 (4/12/2019) |
| 5 | American Heritage | A | Thompson, Kenya | Barrett, Jadae | Rhett, Morgan | Dyer, Thalia | 3:51.92 | FHSAA 2A District 15 (4/13/2019) |
| 6 | Cardinal Gibbons | A | Paul, Anne | Toulemonde, Emma | Thomas, Tori | Jones, Aniyah | 3:59.42 | FHSAA 2A District 15 (4/13/2019) |
| 7 | Rockledge | A | Bostick, Faith | Buckmaster, Tamara | Dawn, Kamari | Joyce, Victoria | 4:05.64 | FHSAA 2A District 13 (4/12/2019) |
| 8 | Suncoast | A | Coleman, Alicia | Smith, Latasha | Emerson, Hanna | Menelas, Ernesha | 4:10.81 | FHSAA 2A District 14 (4/08/2019) |