| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Oak Hall | A | Brown, Kesean | Gill, Jake | Holloway II, Michael | Kaleel, Stephen | 44.99 |
| 2 | Northside Christian | A | Bolds, Jordan | Bolds, Joel | Knox, Jack | Roberts, Andrew | 44.67 |
| 3 | First Baptist (Naples) | A | Fletcher, Ethan | Casey, Thomas | Edie, Austin | Panzarella, Jacob | 43.41 |
| 4 | Admiral Farragut | A | Eubanks, Courtney | Smith, Cameron | Mott, Martel | Reaves, Psaveon | 42.74 |
| 5 | St. John Neumann | A | DeSantis, Daniel | Mathieu, Justin | Raiger, John Paul | Myrin, Alexander | 43.00 |
| 6 | Chiefland | A | Corbin, Ty | Williams, Kirk | Jenkins, L.J | Rutledge, Jalen | 44.60 |
| 7 | Cardinal Mooney | A | Powers, Connor | Mayes, Lemeko | Boynes, Brandon | Green, Isaac | 44.98 |
| 8 | P. K. Yonge | A | Miller, Phillip | Mavin, Deontae | Thomas, Jaleal | Ducharme, Del | DNF |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Bonita Springs | A | Saure, Ethan | Sime-Ainsworth, Maximus | Matamoros, Daniel | Piotrowski, Aidan | 46.24 | FHSAA 1A District 8 (4/10/2019) |
| 2 | Chiefland | A | Corbin, Ty | Williams, Kirk | Jenkins, L.J | Rutledge, Jalen | 45.81 | FHSAA 1A District 5 (4/10/2019) |
| 3 | Northside Christian | A | Bolds, Jordan | Bolds, Joel | Knox, Jack | Roberts, Andrew | 44.42 | FHSAA 1A District 7 (4/11/2019) |
| 4 | St. John Neumann | A | DeSantis, Daniel | Mathieu, Justin | Raiger, John Paul | Myrin, Alexander | 43.14 | FHSAA 1A District 8 (4/10/2019) |
| 5 | Carrollwood | A | Sheppard III, Christopher | Hallenbeck, Nicholas | Anthony, Reidel | Quarles, Jr, Shelton | DQ | FHSAA 1A District 6 (4/11/2019) |
| 6 | Canterbury (FM) | A | Muenscher, Tyler | Camarca, Dominic | Royal, Bryson | Barrett, Konner | 45.55 | FHSAA 1A District 8 (4/10/2019) |
| 7 | P. K. Yonge | A | Miller, Phillip | Mavin, Deontae | Thomas, Jaleal | Ducharme, Del | 45.88 | FHSAA 1A District 5 (4/10/2019) |
| 8 | Trenton | A | Fulmer, Jordan | Riess, Jalen | James, Dylan | Wright, Mason | 47.16 | FHSAA 1A District 5 (4/10/2019) |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Tampa Prep | A | Missana, AJ | Dixon, Cowboy | Sardouk, Adam | Ruiz, Tristan | 46.28 | FHSAA 1A District 6 (4/11/2019) |
| 2 | St. Stephens Episcopal | A | Sherrill, Charlie | Labelle, Noah | Hyppolite, Amonde | Clark, Daniel | 45.85 | FHSAA 1A District 7 (4/11/2019) |
| 3 | Calvary Christian (Clr) | A | Celeste, Jaden | Dials, Gabriel | Martin, Kai | Maynor, Kendrick | DQ | FHSAA 1A District 6 (4/11/2019) |
| 4 | Admiral Farragut | A | Eubanks, Courtney | Smith, Cameron | Mott, Martel | Reaves, Psaveon | 43.29 | FHSAA 1A District 7 (4/11/2019) |
| 5 | First Baptist (Naples) | A | Fletcher, Ethan | Casey, Thomas | Edie, Austin | Panzarella, Jacob | 43.54 | FHSAA 1A District 8 (4/10/2019) |
| 6 | Cardinal Mooney | A | Powers, Connor | Mayes, Lemeko | Boynes, Brandon | Green, Isaac | 45.42 | FHSAA 1A District 7 (4/11/2019) |
| 7 | Cambridge Christian | A | White, Ronald | Martinez, Gavin | Schaffer, Dylan | Snider, Preston | 45.86 | FHSAA 1A District 6 (4/11/2019) |
| 8 | Oak Hall | A | Brown, Kesean | Gill, Jake | Holloway II, Michael | Kaleel, Stephen | 46.40 | FHSAA 1A District 5 (4/10/2019) |