| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 24 | BARNES, Iyana | Take Flight Athletics | NT | ||
| 3 | 151 | GORDON, Ce Mya | QUANTUM TRACK CLUB | 37.25 | ||
| 4 | 147 | BOWERS, Janiyah | QUANTUM TRACK CLUB | 33.49 | ||
| 5 | 283 | NEESMITH, Naomi | FLORIDA EAST COAST SUPERSONICS | 30.00 | ||
| 6 | 78 | EDGECOMBE, Jayla | E.O.T.O. ELITE | 32.00 | ||
| 7 | 170 | THOMAS, Kamya | QUANTUM TRACK CLUB | 34.41 | ||
| 8 | 122 | EVANS, Xaniah | Space Coast Striders | NT |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 3 | 296 | HARDISON, Diarri | E.O.T.O. ELITE | NT | ||
| 4 | 83 | GONZALEZ, Mikelya | E.O.T.O. ELITE | NT | ||
| 5 | 18 | WALLACE, Za'Niya | Riviera Beach Chargers | NT | ||
| 6 | 32 | FISH, Reed | Take Flight Athletics | NT |