| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 37 | JENKINS, Laila | Take Flight Athletics | NT | ||
| 2 | 232 | RIGGINS, Kamaya | I.R.E. Track Club | 28.60 | ||
| 3 | 125 | HAWKINS, Shadae | Space Coast Striders | 26.50 | ||
| 4 | 260 | ROCHELLE, Sanihya | Empire Athletics | 26.18 | ||
| 5 | 269 | GAINES, Sanaii | FLORIDA EAST COAST SUPERSONICS | 26.00 | ||
| 6 | 188 | LOWE, Mikiya | Eastside Xpress | 26.00 | ||
| 7 | 257 | BROWN, Zahmya | Empire Athletics | 26.25 | ||
| 8 | 281 | NEAL, Nierica | FLORIDA EAST COAST SUPERSONICS | 28.20 | ||
| 9 | 238 | STINSON, Davionne | I.R.E. Track Club | 28.90 |