| Lane | # | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|---|
| 1 | 333 | EDWARDS, Tashira | SO | Sprint Tech Track Club | NT |
| 2 | 201 | WILLIAMS, Andreana | JR | Impact Speed Athletics Track Clu | 45.18 |
| 3 | 494 | COOKSEY, Paige | JR | Unattached | 44.83 |
| 4 | 539 | UGARTE, Amaya | JR | Unattached | 42.97 |
| 5 | 25 | CASSELS, Holly | JR | Behind The Athlete | 44.31 |
| 6 | 43 | PHILLIPS, Amaya | SR | Charger Elite | 45.10 |
| 7 | 46 | DEVLIN, Alexis | JR | Dark Horse Athletics | 46.27 |