| PL | JD | Athlete | Yr. | Team | Time | Wind | Pts | HPL | Note |
|---|---|---|---|---|---|---|---|---|---|
| 1 | Mathis, Rayanna | FR | Dr. Phillips | 26.47 | +1.2 | 10 | 1 (1) | ||
| 2 | Dore, Shakaeja | SO | Dr. Phillips | 26.63 | +1.2 | 8 | 1 (2) | ||
| 3 | Carr, Jarvia | FR | Dr. Phillips | 27.52 | +1.2 | 6 | 1 (3) | ||
| 4 | Villanueva Garcia, Cristina | SR | Bishop Moore | 28.16 | +1.2 | 5 | 1 (4) | ||
| 5 | Jackson, Kennedy | SO | Bishop Moore | 28.35 | +1.2 | 4 | 1 (5) | ||
| 6 | Sorensen, Payton | SO | Lake Highland Prep | 28.59 | +1.2 | 3 | 2 (1) | ||
| 7 | Galeano, Daniella | SO | Bishop Moore | 28.89 | +1.2 | 2 | 2 (2) | ||
| 8 | Dorcely, Sagie-May | FR | Bishop Moore | 28.97 | +1.4 | 3 (1) | 28.961 | ||
| 9 | Carrillo, Isabella | SO | Circle Christian | 28.97 | +1.2 | 1 | 2 (3) | 28.969 | |
| 10 | Smith, Julia | SR | Umatilla | 29.81 | +1.4 | 3 (2) | |||
| 11 | Starling , Zariah | FR | Eustis | 29.84 | +0.0 | 5 (1) | |||
| 12 | Lawrence, Jasmyn | SO | Eustis | 29.90 | +0.4 | 6 (1) | 29.897 | ||
| 13 | Covarrubias, Briana | JR | Tavares | 29.90 | +1.2 | 2 (4) | 29.900 | ||
| 14 | Woods, Virginia | FR | Father Lopez | 30.13 | +1.2 | 2 (5) | |||
| 15 | Wilson, Maya | FR | Lake Nona | 30.46 | +1.2 | 2 (6) | |||
| 16 | Kuznof, Kenya | SR | Father Lopez | 30.57 | +1.2 | 1 (6) | |||
| 17 | Williams, Selah | SO | Circle Christian | 30.64 | +1.4 | 3 (3) | |||
| 18 | Foreman, Anna | SO | Geneva School | 30.77 | +1.4 | 3 (4) | |||
| 19 | Bernal, Sofia | FR | Father Lopez | 30.84 | +1.4 | 3 (5) | |||
| 20 | Evans, Maison | SO | Umatilla | 30.93 | +1.4 | 3 (6) | |||
| 21 | Ruiz, Kendall | SR | Lake Nona | 30.95 | +1.4 | 3 (7) | |||
| 22 | Alfreus, Venessa | FR | Eustis | 31.78 | +0.4 | 6 (2) | |||
| 23 | Ribble, Cansas | JR | Tavares | 31.88 | +1.3 | 4 (1) | |||
| 24 | Gill, Alanys | FR | Lake Nona | 32.48 | +0.0 | 5 (2) | |||
| 25 | Serran, Sandra | JR | Tavares | 33.77 | +1.3 | 4 (2) | |||
| 26 | Carrion, Alexis | SO | Winter Springs | 33.87 | +0.0 | 5 (3) | |||
| 27 | Parsons, Claire | FR | Geneva School | 35.12 | +1.3 | 4 (3) | |||
| 28 | Waddell, Haley | SO | Winter Springs | 38.52 | +1.3 | 4 (4) |