| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Smith, Azarian | JR | Bartow | 29.07 | Windermere Meet of Champions 2019 |
| 2 | Leatherwood, Jakira | SO | Ridge Community | 28.06 | 44th Annual Eagle Invitational |
| 3 | Marrero Aquino , Loreine | SR | Mulberry | 28.00 | |
| 4 | Whitehurst, Azaria | 8 | Fort Meade | 26.50 | |
| 5 | Anderson , Shamalrea | SO | Fort Meade | 26.50 | |
| 6 | Guillaume, Gwen | SO | Ridge Community | 28.01 | 44th Annual Eagle Invitational |
| 7 | Groomes, Ajaida | FR | Lake Region | 28.62 | George Jenkins Invitational |
| 8 | Head, Zaniah | JR | Mulberry | 29.84 | Berkeley Track and Field Invitational |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Campbell, Davena | SO | Frostproof | 31.24 | |
| 2 | Fuery, Trinity | FR | Bartow | 30.72 | Auburndale Bloodhounds Preseason Track and Field Classic |
| 3 | Nichols, Maliyah | SO | Fort Meade | 30.60 | |
| 4 | McPlatt, Serena | SR | Mulberry | 30.00 | |
| 5 | Baker, Tre'Niya | JR | Fort Meade | 30.40 | |
| 6 | Cook, Zareale | SR | Mulberry | 30.60 | |
| 7 | Ruskin , Harley | FR | Frostproof | 31.12 | |
| 8 | Hall, na leigha | FR | Lake Region | 31.52 | Lake Region Open |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | James, Oriy'a | JR | Bartow | NT | |
| 3 | Barrett, Shyann | SO | Frostproof | NT | |
| 4 | Butts, Jade | 8 | Frostproof | NT | |
| 5 | Agosto, Alicia | SO | Haines City | NT | |
| 6 | Lopez, Enaylis | JR | Ridge Community | NT | |
| 7 | Outlaw, Andrea | SO | Bartow | NT |