| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | PISELLO, Alaina | Trinity Prep | 5:17.36 |
| 2 | MCDONNELL, Claire | Father Lopez | 5:22.69 |
| 3 | GAYTON, Samantha | Father Lopez | 5:25.00 |
| 4 | PYROS, Rachel | Hagerty | 5:31.13 |
| 5 | CASTRO, Melanie | Winter Park | 5:35.50 |
| 6 | REITZ, Julia | Winter Park | 5:35.60 |
| 7 | WILHELM, Emily | Trinity Prep | 5:40.37 |
| 8 | BLUMENFELD, Sofia | Trinity Prep | 5:40.62 |
| 9 | CRIST, Isabel | First Academy (Orlando) | 5:42.53 |
| 10 | PECK, Mary | Father Lopez | 5:45.00 |
| 11 | JACKSON, Sarah | First Academy (Orlando) | 5:55.00 |
| 12 | VALENCIA, Alisa | Winter Park | 5:55.40 |
| 13 | NUNEZ, Alina | Winter Park | 5:55.60 |
| 14 | GOMEZ, Ana | Winter Park | 5:55.80 |
| 15 | WYANT, Ava | Master's Academy (Oviedo) | 5:56.05 |
| 16 | BARNES, Lauren | Seminole (Sanford) | 6:02.00 |
| 17 | MEDINA-TORRES, Amanda | Hagerty | 6:04.00 |
| 18 | STROMBERG, Allison | Seminole (Sanford) | 6:04.25 |
| 19 | HUPTON, Jasmine | Lyman | 6:07.51 |
| 20 | GOMEZ, Katie | Lake Howell | 6:11.09 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | RAESLY, Ella | Geneva School | 6:15.59 |
| 2 | MILLER, Grace | Geneva School | 6:33.72 |
| 3 | KIRBY, Taylor | Winter Park | 6:35.50 |
| 4 | ULMER, Kate | Orangewood Christian | 7:00.00 |
| 5 | DAVEY, Emma | Trinity Prep | 7:01.30 |
| 6 | MESSEMER, Sarah | Master's Academy (Oviedo) | 7:17.92 |
| 7 | ORTILAZ, Gracie | Orlando Christian Prep | 7:37.54 |
| 8 | KORTMAN, Ava | Trinity Prep | 7:53.20 |
| 9 | ARECHAVALA, Gabriella | Lake Howell | NT |
| 10 | GILBERT, Julianna | Lyman | NT |
| 11 | MAZE, Jacqueline | Lyman | NT |
| 12 | MCANALLY, Lauren | Trinity Prep | NT |
| 13 | NEWMAN, Maria | Master's Academy (Oviedo) | NT |
| 14 | OBERSCHLAKE, Zoe | Lake Howell | NT |
| 15 | PADDOCK, Alli | Master's Academy (Oviedo) | NT |
| 16 | RODD, Erin | Hagerty | NT |
| 17 | STANDER, Sarah | Geneva School | NT |
| 18 | THOMAS, Abby Grace | Geneva School | NT |
| 19 | WITT, Kayla | Lake Howell | NT |