| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | USC-Beaufort |
A | Trinidad | DNS | Sun Conference Track & Field Championship | |||
| 3 | Embry-Riddle |
A | Butts | Roan | Iller | Towers | 3:55.02 | USC Open Indoor |
| 4 | Southeastern U. |
A | Boyd | Simpson | Bigos | Gales | 3:48.00 | |
| 5 | Embry-Riddle |
B | Funke | Jones | Campbell | Saunders | 3:55.02 | USC Open Indoor |
| 6 | A | Spencer | Farwell | Strother | White | 4:02.15 | Duke Invitational | |
| 7 | USC-Beaufort |
B | Thompson | Joseph | DNS |