| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | LOCKRIDGE, Julia | Tampa | 2:14.94 |
| 2 | WEIR, Alexandra | South Florida | 2:15.00 |
| 3 | MAHONY, Aoife | Saint Leo | 2:16.15 |
| 4 | WALSH, Natalie | South Florida | 2:17.00 |
| 5 | BIENZ, Alivia | South Florida | 2:17.55 |
| 6 | BIBLE, Alexis | Tampa | 2:17.57 |
| 7 | ALLMARK, Annie | South Florida | 2:19.45 |
| 8 | ARNOLD, Jirah | South Florida | 2:21.92 |
| 9 | WOLTJER, Emma | Southeastern U. | 2:25.00 |
| 10 | CHRISTIAN, Mariah | Tampa | 2:30.00 |
| 11 | CLODE, Georgia | Saint Leo | 2:30.98 |
| 12 | RIVERA, Jenycha | Southeastern U. | 2:32.00 |
| 13 | BUSH, Brianna | Tampa | 2:32.92 |
| 14 | WELCH, Maddison | Southeastern U. | 2:33.48 |
| 15 | CRAWFORD, Anna | Southeastern U. | 2:35.00 |
| 16 | MELNICOFF, Taylor | Saint Leo | 2:35.00 |
| 17 | MIRANDA, Lizzy | Florida Southern | 2:35.00 |
| 18 | REILLY, Kerry | Saint Leo | 2:35.09 |
| 19 | MACEDO, Kathy | Southeastern U. | 2:38.00 |
| 20 | JOHNSON, Taylor | Tampa | 2:38.00 |
| 21 | WINTON, Amber | Florida Southern | 2:38.65 |
| 22 | LEWANDOWSKI, Caitlin | Saint Leo | 2:53.31 |
| 23 | HANNA, Kate | Florida Southern | 3:08.21 |
| 24 | RHODA, Candace | Florida Southern | 3:10.00 |
| 25 | MOSER, Jessica | Florida Southern | NT |