| Team | Squad | Seed | |
|---|---|---|---|
| 1 | Dunbar | A | 3:20.00 |
| 2 | Auburndale | A | 3:23.33 |
| 3 | George Jenkins | A | 3:25.00 |
| 4 | Hillsborough | A | 3:25.47 |
| 5 | Zephyrhills | A | 3:26.25 |
| 6 | Mitchell | A | 3:28.96 |
| 7 | Plant City | A | 3:29.00 |
| 8 | George Steinbrenner | A | 3:30.00 |
| 9 | Sunlake | A | 3:30.56 |
| 10 | Boca Ciega | A | 3:31.30 |
| 11 | Largo | A | 3:33.16 |
| 12 | Sickles | A | 3:34.25 |
| 13 | Middleton | A | 3:35.00 |
| 14 | East Lake | A | 3:35.01 |
| 15 | Wiregrass Ranch | A | 3:35.31 |
| 16 | Winter Haven | A | 3:39.00 |
| 17 | Tampa Bay Tech | A | 3:40.10 |
| 18 | Forest | A | 3:41.67 |
| 19 | Riverview | A | 3:42.72 |
| 20 | Seffner Christian | A | 3:43.02 |
| 21 | Braden River | A | 3:44.21 |
| 22 | Mulberry | A | 3:45.00 |
| 23 | Robinson | A | 3:45.00 |
| 24 | Riverview (Sarasota) | A | 3:50.02 |
| 25 | Lakewood Ranch | A | 3:50.29 |
| 26 | Pasco | A | 3:53.61 |
| 27 | Freedom (Tampa) | A | 4:00.00 |
| 28 | Lakeland | A | NT |
| 29 | Alonso | A | NT |