| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Jackson, Breyana | SO | Colonial | 1:07.00 | practice |
| 2 | Overby, Nia | FR | Colonial | 1:05.00 | practice |
| 3 | Hall, Aniyah | SO | East River | 1:00.88 | St. Cloud Invitational 2020 |
| 4 | Jones, Hannah | SO | Apopka | 59.72 | 59.72 Metro West Championship 2019 |
| 5 | Brown, Sydni | SO | Lake Nona | 1:00.52 | FHSAA 4A Region 1 and FHSAA 2A Region 2 |
| 6 | Smith, Jalaea | SO | Boone | 1:05.00 | Practice |
| 7 | Schultz, Anamaria | SO | Winter Park | 1:05.99 | Metro East Championship 2019 |
| 8 | DeJesus, Jayline | SO | Colonial | 1:07.00 | St. Cloud Invitational 2020 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Cruz, Sarai | SO | Boone | 1:11.66 | Windermere Meet of Champions 2019 |
| 2 | Henry, Ann Joe | SO | Oak Ridge | 1:09.24 | Lake Brantley Developmental Meet #1 2020 |
| 3 | Bernard, Patley | FR | Winter Park | 1:08.91 | St. Cloud Invitational 2020 |
| 4 | Mapili, Talia | FR | Timber Creek | 1:07.49 | St. Cloud Track Invitational |
| 5 | CHARLES, KAYLANI | FR | Oak Ridge | 1:08.48 | MDC |
| 6 | Hoyos, Valerie | SO | Lake Nona | 1:09.22 | nona |
| 7 | Washington, Joy | FR | Winter Park | 1:10.50 | Orange and Black |
| 8 | Torres Zapata, Tayra | SO | Lake Nona | 1:11.74 | St. Cloud Invitational 2020 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Alas Hernandez, Alejandra | SO | Cypress Creek | 1:19.98 | Metro East Championship 2019 |
| 2 | ALFORD, NYASIA | FR | Oak Ridge | 1:14.44 | Mount Dora Christian Track and Field Meet #2 |
| 3 | Diorio, Rossella | SO | East River | 1:13.15 | Merritt Island Track and Field Invitational |
| 4 | Philips, Cele | SO | Boone | 1:12.06 | Boone HS Quad 2019 |
| 5 | Euceda, Ariana | SO | Cypress Creek | 1:12.23 | CC |
| 6 | Bakarat, Joudi | SO | Timber Creek | 1:13.48 | Larry Kelly Invitational |
| 7 | Ughulu, Shanna | SO | Cypress Creek | 1:15.43 | CC |
| 8 | Rai, Ganesa | FR | Freedom (Orlando) | 1:24.91 | Tohopekaliga Developmental Meet |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | Lopez, Sophia | FR | East River | NT | |
| 4 | Henderson, Sydney | SO | University (Orlando) | 1:25.32 | 3rd Annual Windermere Meet of Champions Classic |
| 5 | Tometi , Alyssa | FR | University (Orlando) | NT |