| Lane | Team | Squad | Seed |
|---|---|---|---|
| 3 | Florida Southern |
B | DNS |
| 4 | Florida Southern |
C | DNS |
| 5 | Florida Southern |
D | DNS |
| 6 | Florida Southern |
A | DNS |
| 7 | Saint Leo |
A | DNS |
| Lane | Team | Squad | Seed |
|---|---|---|---|
| 1 | Lynn |
A | DNS |
| 2 | Embry-Riddle |
B | 3:25.00 |
| 3 | Nova Southeastern |
B | DNS |
| 4 | Embry-Riddle |
A | 3:12.50 |
| 5 | Nova Southeastern |
A | DNS |
| 6 | Florida Tech |
A | NT |
| 7 | Tampa |
A | 3:28.03 |
| 8 | Tampa |
B | 3:36.00 |