| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | South Lake | A | Jones | Moore | Watson | Williams | 1:10.00 | |
| 2 | Poinciana | A | Philip | Davis | Joseph | Davis | DNS | 58.21 Storm Relays |
| 3 | East Ridge | A | Russi | Scott | Badeaux | Udom | 51.27 | FHSAA Class 4A-District 3 |
| 4 | Lakeland | A | Riley | Morse | Chatman | Thomas | DNS | 2019 FHSAA State Track & Field Championships |
| 5 | Bartow | A | Nesbitt | Clay | Paes | Jones | DNS | 51.24 Polk County Championship 2019 |
| 6 | Bartram Trail | A | Moore | Sutherland | Taylor | Dysart | 55.00 | |
| 7 | George Jenkins | A | Boehringer | Deleanibus | Flowers | Terrell | 1:00.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Lake Wales | A | Cenatus | Griffin | Ingram | Meeks | DNS | |
| 4 | Winter Haven | A | Gornail | Hazlett | Joseph | Perry | NT | |
| 5 | Lake Region | A | Alcocer | Baldwin | Brutus | Collier | DNS |