| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | East Ridge | A | Forbes | Levarity | Jeremiah | Valentino | NT | |
| 2 | Bartow | A | Graydon | Williams | Hudson | Brown | 3:36.21 | 3:36.21 Bob English Relays 2019 |
| 3 | Haines City | A | Echezarria | Loriston | Scott | baker | 3:26.00 | |
| 4 | West Orange | A | Bellet | Goodman | Delitz | Nicolas | 3:23.00 | |
| 5 | George Jenkins | A | Runcie | Jean | Rupe | Rupe | 3:25.00 | |
| 6 | Bartram Trail | A | Stiles | Phillips | Goodrich | Barnett | 3:35.00 | |
| 7 | Winter Haven | A | Carter | Richardson | Vasquez-Juarez | Ruiz | 3:39.00 | |
| 8 | Lake Gibson | A | Arirriga | Diodonet | Lang | Borja | NT |