| Lane | Team | Squad | Seed |
|---|---|---|---|
| 1 | Markham Woods Middle School | A | NT |
| 2 | Rock Lake Middle School | A | 1:05.77 |
| 3 | Sanford Middle School | A | 59.01 |
| 4 | Lawton Chiles Middle | A | 55.00 |
| 5 | Jackson Heights Middle School | A | 57.32 |
| 6 | Greenwood Lakes Middle School | A | DQ |
| 7 | South Seminole Middle School | A | NT |
| 8 | Galileo School for Gifted Learni | A | NT |
| Lane | Team | Squad | Seed |
|---|---|---|---|
| 3 | Teague Middle School | A | NT |
| 4 | Sanford Millennium Middle School | A | NT |
| 5 | Milwee Middle School | A | DNS |