| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | McKeel | A | Hunter | Henry | Veloso | Williams | 48.32 | Bob English Relays 2020 |
| 3 | George Jenkins | A | Salter | Crowe | Hargrove | Poe | 44.50 | |
| 4 | Lakeland | A | Gant | Smith | Dennis | Brooks | 43.27 | Bob English Relays 2020 |
| 5 | Lake Gibson | A | Boykins | Randoph | McCall | Wallace | 43.58 | Bob English Relays 2020 |
| 6 | Victory Christian (LL) | A | Jean | Poe | Powell | Shaw | 44.64 | Bob English Relays 2020 |
| 7 | Tenoroc | A | Felton | Peterson | Reid | Coursey | DNS | Poinciana Relays |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Kathleen | A | Pinion | Coppins | Jackson | Copeland | NT | |
| 4 | Vanguard (LW) | A | McDonald | Kabala | Jagielski | Pawelka | DNS | |
| 5 | Lakeland Christian | A | Williams | Davis | Oxendine | Smith | DNF |