| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Windermere Prep | A | 4:00.00 | |
| 4 | Mount Dora | A | DQ | |
| 5 | Winter Park | A | 3:44.58 | Metro East Frosh Soph Championship 2020 |
| 6 | Cypress Creek | A | 3:45.23 | |
| 7 | Boone | A | 3:55.00 | |
| 8 | Wekiva | A | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Edgewater | A | DNS | 3rd Annual Windermere Meet of Champions Classic |
| 2 | Freedom (Orlando) | A | 3:37.36 | Embry-Riddle Spike's Classic (high School) |
| 3 | Sarasota | A | 3:32.38 | Sarasota County Championships |
| 4 | Winter Haven | A | 3:29.28 | FHSAA 3A District 8 and 4A District 5 |
| 5 | First Academy (Orlando) | A | DNS | 2019 FHSAA State Track & Field Championships |
| 6 | Olympia | A | 3:28.82 | Metro West Championship 2019 |
| 7 | George Jenkins | A | 3:32.00 | |
| 8 | Lake Howell | A | 3:33.22 | FHSAA 4A Region 1 and FHSAA 2A Region 2 |
| 9 | Apopka | A | 3:40.00 |