| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Hendrickson, Michael | SO | Sarasota | 43.52 | Sarasota County Championships |
| 2 | Staas, Daniel | SR | Venice | 42.83 | Charlotte Invitational |
| 3 | Collins, Keandre | SR | Sarasota | 38.27 | UNF Pre-State High School Meet |
| 4 | Bennett, Dominic | JR | Sarasota | 41.50 | |
| 5 | Hill, Chase | JR | Bishop Verot | 42.96 | FHSAA Class 2A-District 12 |
| 6 | Gordon, Dejuan | JR | Booker | 43.52 | Sarasota County Championships |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Smith, Dustin | SR | Venice | 47.61 | Charlotte Invitational |
| 2 | Hodo, Jaheim | JR | Braden River | 45.44 | East Lake Early Bird Invitational |
| 3 | Lawes, Biston | JR | Charlotte | 43.92 | South Fort Myers Wolfpack Invitational |
| 4 | White, Isaac | JR | Charlotte | 45.00 | |
| 5 | Meyer, Daniel | SR | Venice | 46.37 | East Lake Early Bird Invitational |
| 6 | Robinson Jr, Norvell | JR | North Port | 47.73 | East Lake Early Bird Invitational |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Grover, Landon | FR | Braden River | 55.11 | Charlotte Invitational |
| 2 | Flerlage, Cooper | FR | Cardinal Mooney | 51.10 | Larry Richardson Memorial Invite |
| 3 | Rue, David | Bishop Verot | 49.19 | Bear Invitational | |
| 4 | Binns, Dale | SO | North Port | 50.87 | |
| 5 | Parks, Dylan | SO | Cardinal Mooney | 51.67 | Sarasota County Championships |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | Contorno , Tyler | FR | Bishop Verot | NT | |
| 3 | Feliz, Justin | SO | Braden River | 1:04.49 | Charlotte Invitational |
| 4 | Jean-Louis, Marc | JR | Cardinal Mooney | NT |