| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Menendez | A | Youmas | Miller | Tompkins | Harris | DNS | |
| 2 | Ridgeview | A | Osman | Roy | Whaley | Duchene | 4:32.16 | FHSAA 2A Region 1 and FHSAA 1A Region 1 |
| 3 | Fernandina Beach | A | Culley | Serrano | Garner | Vining | DNS | FHSAA 2A Region 1 and FHSAA 1A Region 1 |
| 4 | Braddock | A | Adjouadi | Bencosme | Astorga | Astorga | 4:27.52 | Miami Hurricane Invitational |
| 5 | Orange Park | A | Saunders | Francois | Johnson | Williams | 4:29.07 | Bob Hayes Invitational |
| 6 | Middleburg | A | Burt | Nolan | Lynn | Mussante | 4:31.75 | Clay County Championships |
| 7 | St. Augustine | A | Barton | Geter | Lee | Fluman | 4:44.39 | Ancient City Invitational |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Bartram Trail | A | Bori | Henley | Ivey | Bachmann | 4:27.40 | |
| 2 | Trinity Christian (Jax) | A | Bozeman | Slebos | Gugia | Bawroski | 4:23.24 | FHSAA 2A Region 1 and FHSAA 1A Region 1 |
| 3 | Nease | A | Thompson | Cuevas | Murphy | Dodier | 4:17.43 | FHSAA 3A Region 1 |
| 4 | Episcopal | A | Hardwick | McEvoy | Atcherson | Rawls | DNS | Creekside High School Friday Knight Invite 2019 |
| 5 | University Christian | A | Cleveland | Langi | Bell | Cleveland | DNS | Bolles Bulldog Classic |
| 6 | St. Johns Country Day | A | Sage | Moody | Johnson | Sage | DNS | FHSAA 1A District 4 |
| 7 | Creekside | B | Petrick | Iliff | Rose | Graser | 4:25.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Columbia | A | Jerkins | Dixon | Steele | Pope | 4:13.29 | FHSAA 3A Region 1 |
| 2 | Oakleaf | A | Pearson | Silkert | Turner | Jackson | 4:06.15 | FHSAA 4A District 1 |
| 3 | Creekside | A | Skoglund | Till | Harvey | Rice | 4:05.14 | Ancient City Invitational |
| 4 | Raines | A | Kirkland | Noisette | Reid el | Blue | DNS | 2019 FHSAA State Track & Field Championships |
| 5 | Paxon | A | Strickland | Berry | Gill | Stanford | DNS | Gateway Conference Championship |
| 6 | Fleming Island | A | Amsden | Richards | Thompson | Thompson | 4:05.86 | FHSAA 4A Region 1 and FHSAA 2A Region 2 |
| 7 | Chiles | A | Churchill | McNeill | Churchill | Molen | 4:11.00 |