| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | 
|---|---|---|---|---|---|---|---|---|
| 3 | Oakleaf | A | Middleton | Ledgister | Culver | Philogene | NT | |
| 4 | Menendez | A | Giammanco | Harris | Handley | Gilkey | NT | |
| 5 | Bishop Kenny | A | Haywood | Mitchum | Johnson | Shultz | DNS | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | 
|---|---|---|---|---|---|---|---|---|
| 1 | Yulee | A | Bergeron | Clarke | McDaniel | Mumbauer | 2:03.70 | Nease Relays | 
| 2 | Episcopal | A | Selmer | Wright | Atcherson | Rawls | DNS | Bolles Middle School Invitational | 
| 3 | Nease | A | Thompson | Murphy | Dodier | Toussaint | 1:51.30 | Nease Relays | 
| 4 | Creekside | A | Pitts | Mills | Mills | Till | 1:44.75 | Florida Relays | 
| 5 | Columbia | A | Jerkins | Dixon | Pope | Collins | DNS | Florida Relays | 
| 6 | Fleming Island | A | Pogue | Hayes | Norton | George | DNS | Nease Relays | 
| 7 | Orange Park | A | Johnson | Gilford | Saunders | Francois | DNS | Ridgeview Panther Invitational |