| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | BULFORD-TROTT , Keturah | Montverde Academy | 57.50 |
| 2 | DOUGLAS, Domanique | Dr. Phillips | 1:00.71 |
| 3 | MCMULLEN, Madison | Montverde Academy | 1:01.00 |
| 4 | CARR, Jarvia | Dr. Phillips | 1:01.79 |
| 5 | SAFFORD, Lourdes | Dr. Phillips | 1:01.80 |
| 6 | JACKSON, Kennedy | Bishop Moore | 1:02.17 |
| 7 | TOMLINSON, Taylor | Bishop Moore | 1:03.72 |
| 8 | BASS, Taneria | Flagler Palm Coast | 1:04.52 |
| 9 | ADEMU JOHN, Adeena | Windermere | 1:05.56 |
| 10 | TREJO, Briana | Tavares | 1:05.96 |
| 11 | FOSTER, Ava | Tavares | 1:06.47 |
| 12 | MULLIGAN, Mackenzie | Tavares | 1:08.17 |
| 13 | DESAI, Kayla | Lake Highland Prep | 1:10.07 |
| 14 | GONZALEZ, Marisa | Flagler Palm Coast | 1:10.11 |
| 15 | EARLY, Hayden | Bishop Moore | 1:11.20 |
| 16 | TAYLOR, Tamaya | Windermere | 1:11.57 |
| 17 | BOVINO, Hailey | Flagler Palm Coast | 1:15.09 |
| 18 | GRABHER, Ellyana | Windermere | 1:17.43 |
| 19 | EMILCAR, Laura | Montverde Academy | NT |