| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Central Middle School | A | Boatman | Whipple | Mize | Lopez | DNS | Unverified: |
| 2 | Rockledge Kennedy MS | A | 1:01.00 | Unverified: Space Coast | ||||
| 3 | Emma Jewel Charter Academy | A | Corbitt | Mann | Thompson | Jenkins | 58.50 | Unverified: |
| 4 | Central Middle School | B | 54.39 | Verified | ||||
| 5 | Palm Bay Southwest Middle School | A | Wallace | Ellieyanna | Brown | Brown | 58.21 | Verified |
| 6 | Cocoa HS | A | 1:01.00 | Unverified: | ||||
| 7 | Andrew Jackson MS | A | 1:01.00 | Unverified: Brevard County Middle School Cha | ||||
| 8 | West Shore HS | A | Mannix | Murphy | Rukab | Sorgenfrei | 1:03.00 | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Lyndon B. Johnson Middle School | A | 1:09.00 | Unverified: | ||||
| 3 | Delaura Middle School | A | 1:06.00 | Unverified: | ||||
| 4 | Ronald McNair Middle School | A | WILLIAMS | Crooms | BAKER | MERRICK | 1:04.00 | Unverified: |
| 5 | Thomas Jefferson Middle School - | A | 1:04.60 | Unverified: | ||||
| 6 | Stone Magnet Middle School | A | 1:09.00 | Unverified: | ||||
| 7 | Emma Jewel Charter Academy | B | Bryant | DNS | Unverified: |