| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Perez, Abigail | SR | Land O'Lakes | 28.31 | George M. Steinbrenner Track and Field Invitational 2019 |
| 2 | Stinyard, Nylah | JR | Zephyrhills | 28.06 | 28.06 Cypress Creek Invitational 2019 |
| 3 | Bates, Lauren | SR | Land O'Lakes | 27.68 | Cypress Creek QUAD #2 2019 |
| 4 | Gillespie, Delcina | SR | Cypress Creek (Wesley Chapel) | 27.52 | Sunshine Athletic Conference East 2019 |
| 5 | Llanes, Maya | SO | Zephyrhills | 27.60 | 27.60 Sunshine Athletic Conference East 2019 |
| 6 | Lacey, Ashleigh | JR | Cypress Creek (Wesley Chapel) | 27.73 | Cypress Creek QUAD #2 2019 |
| 7 | Pollicita, Alyssa | SO | Cypress Creek (Wesley Chapel) | 28.10 | FHSAA 2A District 9 |
| 8 | Crowley, Sy | SO | Weeki Wachee | 28.70 | Hernando Kiwanis Track and Field Invitational |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Pickett, Jade | FR | Zephyrhills | NT | |
| 2 | Vo, Lauren | JR | Zephyrhills | NT | |
| 3 | Difrank, Sherrie | JR | Weeki Wachee | 30.86 | |
| 4 | Howard, Nadine | JR | Land O'Lakes | 29.33 | 29.33 Sunshine Athletic Conference East 2019 |
| 5 | Wilner, Asia | JR | Zephyrhills | 30.20 | |
| 6 | Miller, Zada | FR | Weeki Wachee | NT | |
| 7 | Halyard, Jai' neicea | JR | Zephyrhills | NT | |
| 8 | Oliver, Trinity | FR | Zephyrhills | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | Rowden, Ciara | FR | Weeki Wachee | NT | |
| 3 | Rober, Cassandra | SO | Weeki Wachee | NT | |
| 4 | Desrameaux, Alexandria | SO | Cypress Creek (Wesley Chapel) | NT | |
| 5 | DiFrank, Rachel | FR | Weeki Wachee | NT | |
| 6 | Malhotra, Mishka | FR | Land O'Lakes | NT | |
| 7 | Patituce, Lana | FR | Zephyrhills | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | Graves, Taylor | FR | Land O'Lakes | NT | |
| 4 | Roman, Zoemarie | FR | Zephyrhills | NT | |
| 5 | Hartman, Maggie | SO | Land O'Lakes | NT |