| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Cocoa Beach HS | A | 1:07.00 | Unverified: | ||||
| 4 | Ronald McNair Middle School | A | CLARK | JONES | BENKEN | ESPOSITO | 1:03.00 | Unverified: Benken/Esposito/Jones/Clark |
| 5 | Hoover Middle School | A | BUCK | SCHOOK | DIXON | BURR | 1:04.00 | Unverified: |
| 6 | Central Middle School | A | Lindie | Brown | Hansen | Wise | 58:03.00 | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Kennedy Middle School | A | Mitchell | Hassanali | Hall | Birkenstock | 1:02.00 | Unverified: Space Coast |
| 2 | Delaura Middle School | A | 1:00.56 | Unverified: | ||||
| 3 | Stone Magnet Middle School | A | Herman | Mick | SAWATSKY | Spence | 1:00.00 | Unverified: 1:00 |
| 4 | West Shore HS | A | King | Sharma | Green | Riopelle | 54.00 | Unverified: |
| 5 | James Madison Middle School | A | Agee | Row | Mitchell | Hawkins | 59.96 | Unverified: |
| 6 | Thomas Jefferson Middle School - | A | Sanchioni | Gruner | Getchell | Husain | 1:00.00 | Unverified: |
| 7 | Palm Bay Southwest Middle School | A | LEE | HILLIARD | DIVYA | JONES | 1:02.00 | Unverified: CENTRAL |
| 8 | Andrew Jackson MS | A | Mitchell | Miller | Williams | Higgins | 1:03.00 | Unverified: |