| Lane | # | Athlete | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | 10 | WESTAWAY, Jenna | London Western | 4:06.90 | |
| 2 | 3 | GRANADOS, Ayla | Hoka One One Aggies | 4:13.40 | |
| 3 | 1 | LUCKI, Alexandra | Toronto west athletics | 4:13.50 | |
| 4 | 12 | ANDREWS, Mackenzie | Unattached | 4:20.00 | |
| 5 | 23 | JUREW, Anna | CPTC-Tracksmith | 4:26.00 | PACER |
| 6 | 24 | BRUMLEY, Briar | CPTC-Tracksmith | 4:28.00 | |
| 7 | 13 | RYAN, Kaitlin | Unattached | 4:29.00 | |
| 8 | 25 | FISHER, Laurel | CPTC-Tracksmith | 4:32.00 | |
| 9 | 26 | ASARO, Amanda | CPTC-Tracksmith | 4:37.00 | |
| 10 | 22 | BELL, Rolanda | CPTC-Tracksmith | NT | PACER |
| 11 | 17 | KOSICHENKO, Olga | CPTC-Tracksmith | NT | PACER |
| 12 | 8 | MELCHER, Annette | US Air Force WCAP-CSTC Elite | NT | PACER |