| Lane | Athlete | Team | Note |
|---|---|---|---|
| 1 | GONZALEZ, Tianna | Haines City | |
| 2 | LEGER, Derionan | Haines City | |
| 3 | WIGGINS, Vivion | Bartow | |
| 4 | BROWN, Lliah | Lake Region | |
| 5 | CASTILLO, Melanie | Ridge Community | |
| 6 | CESAIRE, Jorane | Ridge Community | |
| 7 | KAZMIER, Hannah | Bartow |
| Lane | Athlete | Team | Note |
|---|---|---|---|
| 1 | HENDERSON, Jasmine | Sickles | |
| 2 | GRAYDON, Ta'Metria | Bartow | St. Cloud Invitational 2020 |
| 3 | JACOBSON, Ashlynn | Newsome | |
| 4 | NIELSEN, Cheylie | Lakeland | |
| 5 | RUSH-HAMILTON, Hailee | Charlotte | Charlotte/North Port Preseason Classic |
| 6 | HARTMAN, Sommer | Sickles | 50th Nash Higgins Relays 2020 |
| 7 | GALLARD, Jamari | Ridgeview Global Studies | |
| 8 | AZOR, Alisha | Haines City |
| Lane | Athlete | Team | Note |
|---|---|---|---|
| 1 | CULVER, Madison | Alonso | Kincaid Classic 2021 |
| 2 | SCHULDT, Gabrielle | Newsome | IMG Academy Invitational |
| 3 | STEPHENS, Haillie | Newsome | |
| 4 | JOSHWAY, Daria | Auburndale | |
| 5 | JAMERSON, Kenya | Winter Haven | Brian Jaeger Elite Classic 2020 |
| 6 | SERRANO, Xenia | George Jenkins | |
| 7 | QUEVEDO, Alex | Lakeland | |
| 8 | CASARELLA, Karlee | Charlotte |
| Lane | Athlete | Team | Note |
|---|---|---|---|
| 1 | GUTCHER, Carley | Gaither | Kincaid Classic 2021 |
| 2 | HART, Paris | Kathleen | |
| 3 | DECOSEY, Kiavian | Lakeland | |
| 4 | WILSON, I'Asia | Kathleen | |
| 5 | BRAZELL, Serenity | Wharton | |
| 6 | RAQUET, Alexandra | Sickles | Leto 2/10/2020 |
| 7 | WRIGHT, Jalayla | George Jenkins | |
| 8 | KELLY, Jo'Kierrea | Kathleen | 1:03.99 Polk County Championship 2019 |