| Lane | Team | Squad | Note |
|---|---|---|---|
| 1 | Ridge Community | A | |
| 2 | Haines City | A | 51.86 Polk County Championship 2019 |
| 3 | Alonso | A | 2019 FHSAA State Track %26 Field Championships |
| 4 | Dr. Phillips | A | 2019 FHSAA State Track & Field Championships |
| 5 | Lakeland | A | 2019 FHSAA State Track & Field Championships |
| 6 | George Jenkins | A | |
| 7 | Newsome | A | Spoto Quad Meet 4 |
| 8 | Ridgeview Global Studies | A |