| Lane | Team | Squad | Note |
|---|---|---|---|
| 1 | Alonso | A | FHSAA 4A District 6 |
| 2 | George Jenkins | A | |
| 3 | Lake Region | A | |
| 4 | Sickles | A | 8:47.48 Jesuit Tri-Meet 2-27-19 |
| 5 | Wharton | A | Wharton Wildcat Invitational |
| 6 | Winter Haven | A | Poinciana Relays |
| 7 | Charlotte | A | |
| 8 | Bartow | A | 9:28.20 Bob English Relays 2019 |
| 9 | Newsome | A | |
| 10 | Haines City | A | Storm Preview |
| 11 | Dr. Phillips | A | Storm Preview |
| 12 | Lakeland | A |