| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | Simons, Jacob | Atlantic Coast | 57.32 | Ed White Mini Meet 2 |
| 2 | Ward, Khalil | Sandalwood | 55.24 | FHSAA 4A District 1 |
| 3 | Daniel, Cameron | Atlantic Coast | 54.74 | Ed White Mini Meet 2 |
| 4 | Bell, Aaron | Terry Parker | 49.00 | |
| 5 | Terry, David | Sandalwood | 51.64 | Donell Butler Relays |
| 6 | Williams, Tyrek | Terry Parker | 55.00 | |
| 7 | Rodgers, Julian | Ribault | 56.50 | |
| 8 | Valsaint, Isaiah | Westside | 57.34 | Ed White Mini Meet 1 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | Thompson, Zeki | Paxon | NT | |
| 2 | Kent, Titus | Westside | NT | |
| 3 | Toson, Nathan | Ribault | NT | |
| 4 | Williams, Darrell | Westside | 1:00.93 | Donell Butler Relays |
| 5 | Flowers, Nicholas | Paxon | 1:01.02 | Donell Butler Relays |
| 6 | Maron, Jeranthony | Fletcher | NT | |
| 7 | Edwards, Demetrius | Sandalwood | NT | |
| 8 | Honore, Jaden | Paxon | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | Barbee, Ezra | Atlantic Coast | NT | |
| 2 | Session, Keivon | Ribault | NT | |
| 3 | Fox, Colin | Fletcher | NT | |
| 4 | Whitley, Jacob | Wolfson | NT | |
| 5 | McCrae, Antwon | Westside | NT | |
| 6 | Anderson, Alonzo | Paxon | NT | |
| 7 | Carter, Shannon | Atlantic Coast | NT | |
| 8 | Tankard, Kaleb | Sandalwood | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | Joseph, Bernadson | Terry Parker | NT | |
| 3 | Grabau, Luca | Wolfson | NT | |
| 4 | Bailey, Sean | Wolfson | NT | |
| 5 | Sheppard, Jerrell | Fletcher | NT | |
| 6 | Hutto, Josh | Fletcher | NT | |
| 7 | Dorsey Jr, Char ron | Terry Parker | NT |