| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | Brown, Zavontray | Ribault | 47.50 | |
| 4 | Garrison, King | Atlantic Coast | 45.29 | Ed White Mini Meet 2 |
| 5 | Davis Jr, Kevin | Atlantic Coast | 39.40 | |
| 6 | Sinclair, Devon | Terry Parker | 44.63 | Ed White Mini Meet 1 |
| 7 | Jackson, Jacobi | Terry Parker | 46.54 | Ed White Mini Meet 1 |
| 8 | Backmon, X'zavier | Ribault | 48.50 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | Ortega, Eljandro | Fletcher | NT | |
| 4 | Davis, Zarie | Atlantic Coast | NT | |
| 5 | Alvies , Bruno | Atlantic Coast | NT | |
| 6 | Daniels, Jerrell | Fletcher | NT | |
| 7 | Houston, Za'vion | Ribault | NT | |
| 8 | Patterson, Samuel | Fletcher | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 6 | Washington, Christian | Sandalwood | NT | |
| 7 | Sheppard, Jerrell | Fletcher | NT | |
| 8 | Sutton, Romello | Sandalwood | NT |