| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Terry Parker | A | Wright | Joseph | Bradley | Sinclair | 47.20 | |
| 2 | Fletcher | A | Taylor | Riley | Patterson | Schrock | 44.42 | Ed White Mini Meet 2 |
| 3 | Paxon | A | Morris | Miller | Escoffrey | Henson | 43.47 | Florida Relays |
| 4 | Sandalwood | A | Collier | Blount | Griffin | Hillman | 42.00 | FHSAA Class 4A-District 1 |
| 5 | Atlantic Coast | A | Blake | Bryant | Seaborn | Davis Jr | 42.89 | 2018 FHSAA State Track & Field Championships |
| 6 | Ribault | A | Hall | Rodgers | Robinson | Williams | 43.50 | |
| 7 | Wolfson | A | Williams | Jenkins | Gazaleh | Maxwell | 45.48 | 36th Wolfpack Classic |