| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Seminole (Sanford) | A | Stephens | McKenzie | Kelly | Givens | 1:37.00 | |
| 3 | Hagerty | A | Farwig | Felix | Schilling | Garan | 1:32.50 | |
| 4 | Lake Brantley | A | Vielot | Fourcand | Perkins | Wright | 1:30.00 | |
| 5 | Seabreeze | A | Willis | Pacheco | Campbell | Allison | DNS | |
| 6 | Timber Creek | A | Carillo | Perez | Roman | Joyce | 1:34.00 | |
| 7 | Oviedo | A | Esquivel | Haddad | Metz | Adams-Brace | 2:00.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | East River | A | Dumas | Simpson | Santiago | Shah | NT | |
| 4 | Lyman | A | March | Leon | Nick | Rodrigez | DNS | |
| 5 | Lake Howell | A | Roberts | Lawrence | McDonald | Curry | NT |