| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | Clair, Nichoia | Bayside | 18.50 | 2/16 Heritage Meet |
| 2 | Barrett, Blyss | Bayside | 17.97 | 2/16 Heritage Meet |
| 3 | Muncey, Bethany | Heritage | 17.87 | |
| 4 | Lewis, Mia | Cocoa | 15.80 | |
| 5 | Daniels, Jiselle | Bayside | 17.58 | |
| 6 | Moury, Hannah | Circle Christian | 17.96 | Mount Dora Christian Track and Field Meet |
| 7 | Saint-Rose, Adelaide | Florida Prep | 18.00 | |
| 8 | Kohler, Sierra | Merritt Island | 18.53 | Holy Trinity Invitational powered by Track Legend Brand |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | Lusk, Maddison | Merritt Island | 20.81 | 2021 Merritt Island Relays |
| 2 | Myers-Santos, Lauren | Merritt Island | 20.16 | Satellite High Reggie Johnson Memorial Classic 2020 |
| 3 | Lherisse, Alayna | Eau Gallie | 19.22 | |
| 4 | Pladdys, Rachel | Florida Prep | 19.00 | |
| 5 | Rosadopadilla, Aidaliz | Palm Bay | 19.22 | 2/16 Heritage Meet |
| 6 | Lawrence , Jada | Rockledge | 19.50 | |
| 7 | Olson-Zapata, Gabriella | Edgewood | 20.55 | MIHS Developmental Meet 2/26/20 |
| 8 | Evans-Johnson, Myracle | Merritt Island | 21.00 | 2021 Merritt Island Relays |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | Sutton, Anijia | Rockledge | NT | |
| 3 | Jones, Madeline | Heritage | 23.10 | |
| 4 | Rosa, Jeslyn | Melbourne | 21.20 | |
| 5 | Bissland, Brooke | Heritage | 23.04 | |
| 6 | Carter, Jasmine | Eau Gallie | 24.62 | 2/16 Heritage Meet |