| Lane | # | Athlete | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | 346 | DAVIS, Ma`kala | Ocoee | 17.74 | Tohopekaliga Invitational 1 |
| 2 | 401 | LAWRENCE, Delicia | Evans | 16.49 | Kowboy Developmental Meet |
| 3 | 414 | SCOTT, Ar'briel | Evans | 15.89 | |
| 4 | 14 | SIMS, Amari | Windermere | 14.99 | |
| 5 | 508 | REESE, Karis | Lake Mary | 15.62 | Lake Mary Invite 2020 |
| 6 | 773 | SOTO, DeMaris | Apopka | 16.33 | 3rd Annual Windermere Meet of Champions Classic |
| 7 | 775 | KERN, Julianna | Apopka | 16.77 | Toho Invitational 2020 |
| 8 | 705 | HENDERSON, Zakhia | East Ridge | 17.91 | Lake Sumter County Championships |
| Lane | # | Athlete | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | 319 | LARRAMORE, Sydney | Ocoee | 21.01 | Tohopekaliga Invitational 1 |
| 4 | 670 | NDUESE-UDOM, Karen | East Ridge | 19.26 | Lake Mary Invite 2020 |
| 5 | 814 | ABRAHAM, Maya | Olympia | 19.59 | Tohopekaliga Invitational 1 |
| 6 | 456 | LAWRENCE, Alton'Nesha | Lake Mary | NT |