| 3A | FHSAA MDE 3A | 41.82 |
| 4A | FHSAA MDE 4A | 41.87 |
| Lane | # | Athlete | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | 160 | BOWEN, Samuel | Hagerty | NT | NT |
| 2 | 438 | HARRIS, Luke | Lake Brantley | 52.71 | Silverhawk Open 2021 |
| 3 | 43 | GILBERT, Aidan | Lake Howell | 45.61 | Silverhawk Open 2021 |
| 4 | 317 | JONES, Markel | Lake Mary | 41.29 | Windermere Meet of Champions 2021 |
| 5 | 626 | DAVIS, David | Seminole (Sanford) | 43.25 | Silverhawk Open 2021 |
| 6 | 458 | RODRIGEZ, Jerome | Lyman | 52.46 | SAC Freshman-Sophmore 2020 |
| 7 | 171 | SMITH, Michael | Hagerty | NT | NT |
| 8 | 631 | ROJAS, Elijah | Seminole (Sanford) | NT | NT |
| Lane | # | Athlete | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | 530 | SLAIN, Ty | Winter Springs | NT | NT |
| 2 | 113 | MORRIS, Brock | Hagerty | NT | NT |
| 3 | 229 | MARSHALL, Blake | Lake Mary | NT | NT |
| 4 | 135 | NGYUYEN, Neiken | Hagerty | NT | NT |
| 5 | 533 | ALBURY, Brett | Winter Springs | NT | NT |
| 6 | 629 | GUTCH, Noah | Seminole (Sanford) | NT | NT |
| 7 | 535 | SERRA, Julian | Winter Springs | NT | NT |
| 8 | 567 | FEQUIERE, Kamau | Seminole (Sanford) | NT | NT |