| 3A | FHSAA MDE 3A | 48.79 |
| 4A | FHSAA MDE 4A | 49.42 |
| Lane | # | Athlete | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | 417 | LOGAN, Chayil | Lake Brantley | NT | NT |
| 2 | 625 | JONES, Natasha | Seminole (Sanford) | NT | NT |
| 3 | 223 | LAWRENCE, Alton'Nesha | Lake Mary | NT | NT |
| 4 | 272 | REESE, Karis | Lake Mary | 49.59 | SAC Freshman-Sophmore 2020 |
| 5 | 92 | JACOB, Neveah | Hagerty | 56.14 | Silverhawk Open 2021 |
| 6 | 12 | LAFORTUNE, Sara | Lake Howell | NT | NT |
| 7 | 119 | COSTA, Casey | Hagerty | NT | NT |