| 1A | FHSAA MDE 1A | 55.05 |
| 2A | FHSAA MDE 2A | 49.50 |
| 3A | FHSAA MDE 3A | 48.79 |
| 4A | FHSAA MDE 4A | 49.42 |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 72 | MCCARTNEY, Ilyana | SR | Faith Christian | NT | |
| 3 | 49 | EVANS-JOHNSON, Myracle | SR | Merritt Island | 55.50 | |
| 4 | 8 | KOHLER, Sierra | SO | Merritt Island | 53.29 | Holy Trinity Invitational powered by Track Legend Brand |
| 5 | 3 | LUSK, Maddison | FR | Merritt Island | 54.55 | |
| 6 | 13 | MYERS-SANTOS, Lauren | SR | Merritt Island | 56.52 |