| 1A | FHSAA MDE 1A | 2:37.50 |
| 2A | FHSAA MDE 2A | 2:48.20 |
| 3A | FHSAA MDE 3A | 2:26.90 |
| 4A | FHSAA MDE 4A | 2:27.30 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | Hildebrand, Maranda | Land O'Lakes | 2:30.44 | Anclote High School Meet One |
| 2 | Poe, Madison | Zephyrhills | 2:31.99 | Cypress Creek Invitational 2019 |
| 3 | D'Piazza, Adia | Mitchell | 2:39.34 | Anclote High School Meet One |
| 4 | Newton, Genavieve | Mitchell | 2:45.03 | River Ridge Meet #2 |
| 5 | Castillo, Aileen | Land O'Lakes | 2:46.66 | Anclote High School Meet One |
| 6 | McFaul, Abbigail | Wiregrass Ranch | 2:48.19 | Cypress Creek Quad 2021 |
| 7 | Erwin, Jessica | Mitchell | 2:48.32 | |
| 8 | Faircloth, Zoe | Cypress Creek (Wesley Chapel) | 2:50.00 | |
| 9 | D'Piazza, Drea | Mitchell | 2:54.24 | Anclote High School Meet One |
| 10 | Hoppe, Grace | Zephyrhills | 2:55.12 | Wharton Wildcat Invite |
| 11 | Paras , Sienna | Wiregrass Ranch | 2:56.78 | Cypress Creek Quad 2021 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | Duquella, Chloe | Wiregrass Ranch | 3:01.90 | Wharton Wildcat Invitational |
| 2 | Bohm, Eleni | Fivay | 3:02.46 | Anclote High School Meet One |
| 3 | Ledin, Sky | Fivay | 3:03.66 | Anclote High School Meet One |
| 4 | Moore, Alaynah | Fivay | 3:04.79 | Anclote High School Meet One |
| 5 | Riner, hope | Zephyrhills | 3:11.24 | Hernando High School Kiwanis Track and Field Meet |
| 6 | Florez, Mackenzie | Zephyrhills | 3:17.99 | Anclote High School Meet One |
| 7 | Green, Jayden | Wiregrass Ranch | 3:28.00 | |
| 8 | Connoy, Grace | Land O'Lakes | NT | |
| 9 | Coll, Cecelia | Bishop McLaughlin | NT | |
| 10 | Adriani, Ava | Land O'Lakes | NT | |
| 11 | Merriett, Camryn | Bishop McLaughlin | NT |